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Table 3 Observed dissociation constants (Kd in μM) for Ypd1 with Sln1-R1

From: Role of the highly conserved G68 residue in the yeast phosphorelay protein Ypd1: implications for interactions between histidine phosphotransfer (HPt) and response regulator proteins

WT

0.94 ± 0.38

G68V

2.9 ± 0.08

G68L

1.3 ± 0.09

G68E

0.5 ± 0.13

G68Q

0.6 ± 0.27

  1. Binding constants and standard deviations were derived from three replicate titrations