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Table 2 Phosphorylation of Ypd1-G68X and phosphorelay activity

From: Role of the highly conserved G68 residue in the yeast phosphorelay protein Ypd1: implications for interactions between histidine phosphotransfer (HPt) and response regulator proteins

Protein

aPhosphorylation (%*)

bPhosphorelay (%*)

WT

100 ± 0

100 ± 0

G68S

94 ± 3.6

81 ± 18

G68A

82 ± 16

80 ± 8.0

G68V

2.5 ± 3.5

0.36 ± 0.6

G68L

0.4 ± 0.6

0.28 ± 0.5

G68E

0.3 ± 0.5

0.08 ± 0.15

G68Q

40 ± 8.3

0.9 ± 1.6

  1. *Values are expressed as a percentage of wild-type. Standard deviations were calculated based on three replicates under similar conditions
  2. aPhosphorylation of Ypd1 mutants from Sln1-R1 as phosphondonor
  3. bPhosphorelay from Sln1-R1 to Ssk1-R2