Fig. 4From: Role of the highly conserved G68 residue in the yeast phosphorelay protein Ypd1: implications for interactions between histidine phosphotransfer (HPt) and response regulator proteinsSaturation binding curve between Ypd1 and Sln1-R1 in a fluorescence binding assay. Fluorescence-based protein binding experiments using Ypd1-T12C~F titrated with Sln1-R1 (♦) or BSA (■). Fluoresceinated Ypd1-T12C (30 pmol) in 1.9 mL of reaction buffer was titrated with Sln1-R1 using a concentration range from 10 nM to 6 μMBack to article page