Table 1 Nucleotide sequence information of qPCR primers and TaqMan probes
From: Identification of the major diacylglycerol acyltransferase mRNA in mouse adipocytes and macrophages
Species | mRNA | Name | Accession no. | Amplicon (bp) | Forward primer (5′ to 3′) | TaqMan probe (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|---|---|---|---|---|
Mouse | Dgat1 | Diacylglycerol acyltransferase 1 | NM_010046 | 61 | CGTGGGCGACGGCTACT | ATCTGAGGTGCCATCG | TGAGCTGAACAAAGAATCTTGCA |
Dgat2 | Diacylglycerol acyltransferase 2 | NM_026384 | 62 | CTGGCTGATAGCTGTGCTCTACTT | CTGGCTGGCATTTG | CTTTCTTGGGCGTGTTCCA | |
Ttp | Tristetraprolin/Zfp36 | NM_011756 | 70 | GGTACCCCAGGCTGGCTTT | AACTCAATATAATCCTGCCTTAGCCTT | ACCTGTAACCCCAGAACTTGGA | |
Adipoq | Adiponectin | NM_009605 | 180 | CCCTCCACCCAAGGGAACTT | TGCAGGTTGGATGGCAGGCATC | TCAGCTCCTGTCATTCCAACAT | |
Rpl32 | 60S ribosome protein L32 | NM_172086 | 66 | AACCGAAAAGCCATTGTAGAAA | AGCAGCACAGCTGGCCATCAGAGTC | CCTGGCGTTGGGATTGG | |
Tung tree | Dgat1 | Diacylglycerol acyltransferase 1 | DQ356680 | 70 | TGGTTGCTCCCACATTGTGT | ACCAGCCAAGTTATCCTCGAACTGCATCC | ACCACCCAACCCTTTCGAA |
Dgat2 | Diacylglycerol acyltransferase 2 | DQ356682 | 80 | TGCATGTCGTGGTGGGTAGA | AAGCAAAATCCACAGCCTACCGCTGAA | TCTCTGTACTTCCGCAACCT | |
Dgat3 | Diacylglycerol acyltransferase 3 | KC993814 | 64 | AAGTGCAGGGACGGTCCTAA | TCAGGGTCAATGCTG | TGGGTGGAGTTGTGATTGGA | |
Rpl19b | 60S Ribosomal protein L19b | FJ362591 | 70 | GCGGAGAATGCGTGTTCTG | CCTGCTGCGCAAATACCGGGAA | CATGTGCTTGTCAATTTTTTTGG |