Effect of IAPP on the proteome of cultured Rin-5F cells

BMC Biochemistry

Table 2 Significantly Down-Regulated Proteins in IAPP Treated Rin-5F Cells. The data were obtained from three independent experiments. To obtain the protein abundance ratio the amount of each protein in the IAPP treated cells was divided by its corresponding amount in the control cells (IAPP untreated cells). The corresponding ratios of all proteins were then averaged between the three experiments. If a specific protein was expressed in either untreated or IAPP treated samples, the missing value was replaced by the smallest amount that was potentially detected by the machine (0.1 femtomole). Differences between IAPP treated and un-treated expression levels are considered to be significant if p ≤ 0.05. To determine the p value two-tailed Student’s t-test was performed, comparing the three individual control amounts with the three corresponding treated samples

Accession #	UniProt Id	Description	Mean ratio (IAPP treated/control)	SD	p Value
IPI00471525	Q68FR9/F1LP72	Uncharacterized protein	0.054	0.076	0.048
IPI00231968	Q5U362	Annexin A4 isoform CRA a	0.097	0.059	0.043
IPI00210357	Q794E4	Heterogeneous nuclear ribonucleoprotein F	0.126	0.040	0.048
IPI00470288	Q9EQS0/P07335	Creatine kinase B type	0.143	0.027	< 0.001
IPI00208215	Q9Z0V6	Thioredoxin dependent peroxide reductase mitochondrial	0.221	0.009	0.034
IPI00387868	O88600/F1LRV4	Heat shock 70 kDa protein 4	0.273	0.021	0.048
IPI00211779	Q63716	Peroxiredoxin 1	0.277	0.001	0.032
IPI00197696	Q0QF43/P04636	Malate dehydrogenase mitochondrial	0.291	0.104	0.013
IPI00201333	D4A0W9	Uncharacterized protein	0.388	0.139	0.050
IPI00190559	Q9EQX9	Ubiquitin conjugating enzyme E2 N	0.453	0.100	0.021
IPI00324893	P63102	14 3 3 protein zeta delta	0.558	0.088	0.001
IPI00200147	P19945	60S acidic ribosomal protein P0	0.678	0.057	0.043
IPI00365935	P83868/B2GV92	Prostaglandin E synthase 3	0.692	0.070	0.005
IPI00200861	P04961	Proliferating cell nuclear antigen	0.877	0.315	0.005
IPI00189925	P54921	Alpha soluble NSF attachment protein	0.005	1.865	0.038
IPI00189989	B2RYK3/P18297	Sepiapterin reductase	0.006	1.089	0.027
IPI00363925	B5DEH4	Uap1l1 protein	0.014	0.234	0.015
IPI00365423	O35511/Q5XI34	Protein phosphatase 2 Formerly 2A regulatory subunit A alpha isoform	0.003	4.101	0.050
IPI00370456	Q4FZT9	26S proteasome non ATPase regulatory subunit 2	0.007	0.491	0.016
IPI00768299	B2GV73/F1LRL8	Actin related protein 2 3 complex subunit 3 Predicted isoform CRA b	0.010	0.798	0.037

ISSN: 1471-2091