Table 1 Proteins Detected in Untreated (Control) and IAPP Treated Rin-5F Cells. Data were obtained from three independent experiments, where each experiment was repeated three times. Data is only reported for proteins detected in all three repeats. F1-F12 are the 12 fractions from OFFGELâ„¢ electrophoresis, separated by pI. Complete data is in Additional file 1: Table S1
From: Effect of IAPP on the proteome of cultured Rin-5F cells
Property | F1 | F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | F10 | F11 | F12 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Theoretical pH range | <  3.6 | 3.6–4.2 | 4.2–4.7 | 4.7–5.3 | 5.3–5.9 | 5.9–6.5 | 6.5–7.1 | 7.1–7.6 | 7.6–8.2 | 8.2–8.8 | 8.8–9.2 | >  9.2 |
# proteins control Ex1 | 29 | 48 | 48 | 16 | 9 | 4 | 53 | 92 | 54 | 26 | 20 | 19 |
Mean pI control Ex1 | 4.8 | 4.7 | 4.9 | 5.2 | 5.3 | 5.8 | 6.6 | 7.2 | 7.6 | 8.2 | 8.3 | 8.6 |
Mean % protein coverage control Ex1 | 29.8 | 32.9 | 35.9 | 26.1 | 31.3 | 26.6 | 24.7 | 26.2 | 29.5 | 24.7 | 27.2 | 28.1 |
# proteins IAPP Ex1 | 33 | 46 | 48 | 53 | 62 | 60 | 70 | 46 | 59 | 15 | 27 | 4 |
Mean pI IAPP Ex1 | 5.0 | 4.8 | 4.9 | 5.1 | 5.6 | 6.2 | 6.4 | 7.1 | 7.5 | 8.2 | 8.5 | 8.8 |
Mean % protein coverage IAPP Ex1 | 27.9 | 33.7 | 29.6 | 29.1 | 25.3 | 28.1 | 26.6 | 25.4 | 27.4 | 18.8 | 31.2 | 27.4 |
# proteins control Ex2 | 106 | 189 | 114 | 180 | 190 | 151 | 214 | 238 | 155 | 192 | 169 | 236 |
Mean pI control Ex2 | 4.9 | 4.9 | 5.1 | 5.4 | 6.0 | 6.4 | 6.8 | 7.0 | 7.6 | 8.0 | 8.1 | 8.5 |
Mean % protein coverage control Ex2 | 23.2 | 29.5 | 31.8 | 30.1 | 29.1 | 24.2 | 24.7 | 24.9 | 29.5 | 24.5 | 26 | 25.9 |
# proteins IAPP Ex2 | 46 | 131 | 149 | 154 | 152 | 223 | 264 | 270 | 223 | 219 | 145 | 187 |
Mean pI IAPP Ex2 | 4.8 | 4.8 | 5.1 | 5.3 | 6.0 | 6.6 | 6.7 | 7.1 | 7.7 | 8.0 | 8.6 | 8.9 |
Mean % protein coverage IAPP Ex2 | 21.6 | 24.3 | 22.9 | 26.2 | 24.5 | 24.6 | 24.5 | 24.2 | 25.2 | 24.4 | 20.7 | 25.7 |
# proteins control Ex3 | 5 | 57 | 52 | 60 | 45 | 64 | 69 | 75 | 30 | 41 | 46 | 0 |
Mean pI control Ex3 | 4.7 | 4.8 | 5.1 | 5.3 | 5.9 | 6.5 | 6.6 | 7.2 | 7.8 | 7.8 | 8.5 | – |
Mean % protein coverage control Ex3 | 24.2 | 32.9 | 29.9 | 23.7 | 22.8 | 26.2 | 25.3 | 25.6 | 27 | 30.8 | 31.4 | – |
# proteins IAPP Ex3 | 15 | 33 | 38 | 56 | 50 | 56 | 74 | 53 | 44 | 58 | 27 | 9 |
Mean pI IAPP Ex3 | 5.0 | 4.8 | 4.9 | 5.3 | 6.0 | 6.6 | 6.5 | 7.0 | 7.8 | 7.9 | 8.4 | 8.9 |
Mean % protein coverage IAPP Ex3 | 25.2 | 34.8 | 28.1 | 30.8 | 23.7 | 27.5 | 23.8 | 26.3 | 27.9 | 32.4 | 33.5 | 31.7 |