Fig. 1
Validation of the feasibility of agarose gel electrophoresis to detect endonuclease activity. A (a) Agarose gel electrophoresis of HO8910 cell lysates with different total protein amounts.: 0.2 μg (1),: 0.4 μg (2),: 0.6 μg (3),: 0.8 μg (4),: 1 μg (5),: 2 μg (6),: 4 μg (7),: 0 μg (8). (b) Curve of the total protein amount (y) and R ratio(x). y = 0.289 x + 0.210, R2 = 0.996. B (a) Agarose gel electrophoresis of 8910 cells at different total numbers in 100 μl Chaps lysis buffer. (b) Histogram of R ratios of HO8910 cells. (c) HO8910 cell’s bacterial colony number. (d) Histogram of HO8910 cell’s bacterial colony number