From: Purification and characterization of α-amylase from Trichoderma pseudokoningii
steps | T. units | T. Protein mg | S.A* | Fold purification | Recovery 100% |
---|---|---|---|---|---|
Crude extract | 605 | 17.3 | 34 | 1 | 100 |
Chromatography DEAE-sepharose | |||||
0.0 M NaCl (A1) | 17.8 | 6.8 | 2.6 | 0.07 | 2.9 |
0.05 M NaCl(A2) | 28 | 0.39 | 71.8 | 2.05 | 4.6 |
0.1 M NaCl (A3) | 31 | 1 | 31 | 0.88 | 5.1 |
0.2 M NaCl (A4) | 422 | 1.04 | 405.7 | 11.6 | 69 |
0.3 M NaCl (A5) | 25 | 1.9 | 13 | 0.37 | 4.1 |
Sephacryl S-200 A4 | 110 | 0.2 | 550 | 15.7 | 18 |