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Table 1 Purification scheme for T. pseudokoningii α-amylase

From: Purification and characterization of α-amylase from Trichoderma pseudokoningii

steps

T. units

T. Protein mg

S.A*

Fold purification

Recovery 100%

Crude extract

605

17.3

34

1

100

Chromatography DEAE-sepharose

0.0 M NaCl (A1)

17.8

6.8

2.6

0.07

2.9

0.05 M NaCl(A2)

28

0.39

71.8

2.05

4.6

0.1 M NaCl (A3)

31

1

31

0.88

5.1

0.2 M NaCl (A4)

422

1.04

405.7

11.6

69

0.3 M NaCl (A5)

25

1.9

13

0.37

4.1

Sephacryl S-200

A4

110

0.2

550

15.7

18

  1. S.A*: Specific activity (total units/ mg protein)