Table 1 Oligonucleotides used in this study for cloning of the genes cur_1714 and cur_1715. The sequences of the primers were derived from the prospective genes cur_1714 and cur_1715 and their flanking regions taken from the genome of C. urealyticum DSM 7109 [13]
Oligonucleotides | Sequence 5’➝ 3’ |
|---|---|
Fwd_1714_XbaI | GTGTCTAGAGACCTACACTCTAGGAGTTTC |
RP pX cur_1714 KpnI | CTGGTACCTTAGAAGCCGAATGCCTG |
RP-cur1714-KpnI pXHis | GCTTAAAGGTACCGAAGCCGAATG |
RevpXMJ19 | CAGACCGCTTCTGCGTTCTG |
Fwd Gst 1714 BamHI | CTGAGGATCCGGTAACGCAAC |
Rev Gst 1714 EcoRI | GACGAATTCTTAGAAGCCGAATGC |
Fwd Gst 1715BamHI | CAGTGGATCCAACGTCGACATG |
Rev Gst 1715 EcoRI | CTAGAATTCCTACTTGTTCTCGG |
Fwd GST Seq | CACTCCCGTTCTGGATAATG |
Rev GST Seq | CACTCCGCTATCGCTACGTGAC |
T7 Promotor | TAATACGACTCACTATAGGG |
M13 reverse | CAGGAAACAGCTATGAC |