Skip to main content
Fig. 2 | BMC Biochemistry

Fig. 2

From: Identification and characterization of smallest pore-forming protein in the cell wall of pathogenic Corynebacterium urealyticum DSM 7109

Fig. 2

a Single-channel recording of a Diph-PC/n-decane membrane in the presence of detergent extract of whole C. urealyticum cells. The aqueous phase contained 1 M KCl, pH 6 and about 50 ng/ml protein extract. The applied membrane potential was 20 mV; T = 20 °C. b Histogram of the probability P(G) for the occurrence of a given conductivity unit observed with membranes formed of 1% Diph-PC dissolved in n-decane. The histogram was calculated by dividing the number of fluctuations with a given conductance unit by the total number of conductance fluctuations in the presence of detergent extracts of whole C. urealyticum cells. Two frequent conductive units were observed for 147 single events taken from 6 individual membranes. The average conductance of the steps corresponding to the left-side maximum was 1.75 nS and that of the right-side maximum was about 4.0 nS. The aqueous phase contained 1 M KCl, pH 6 and about 50 ng/ml protein extract, the applied membrane potential was 20 mV, T = 20 °C

Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us