Skip to main content
Fig. 2 | BMC Biochemistry

Fig. 2

From: Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain

Fig. 2

Verification of antiproliferative activity of APF and as-APF by a cell-based assay. The biological activity of the as-APF and APF peptides was examined by measuring T24 bladder carcinoma cell proliferation using the XTT Cell Proliferation Assay. Metabolically active cells reduce XTT to its formazan derivative, causing a colorimetric change in the medium that can be measured by a spectrophotometer. The relative activities of APF and as-APF are shown and normalized to the control. We observed that both APF and as-APF are biologically active and significantly inhibit cell proliferation (P < 0.0001). The proliferation of T24 cells decreased by 60.16 ± 10.67% after treatment with 20 nM as-APF, while APF (20 nM) reduced proliferation by 47.08 ± 12.88%. The difference in the antiproliferative effect of as-APF and APF was determined to be significant (P = 0.0103). P values were assigned using unpaired t tests with Welch’s correction. The proliferation assay was repeated at least four independent times

Back to article page