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Fig. 5 | BMC Biochemistry

Fig. 5

From: Chemical and structural characterization of α-N-acetylgalactosaminidase I and II from starfish, asterina amurensis

Fig. 5

Effects of tryptophan modification and various monosaccharides on α-GalNAcase and α-galactosidase activities of α-GalNAcase I. a The NBS titration curve of percent residual activity versus the number of tryptophan residues modified was poltted for determination of number of tryptophan residues involved in α-GalNAcase and α-galactosidase activities of α-GalNAcase I. b Effect of various monosaccharide on α-GalNAcase I. α-GalNAcase I was preincubated with various monosaccharide (Man, Gal, Glc, GalNAc and GlcNAc) at 10 mM concentration for 30 min at 25 °C and determined the residual α-galactosidase (□) and α-GalNAcase (■) activities. Data are presented as mean value of three determinations

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BMC Biochemistry

ISSN: 1471-2091

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