Fig. 1From: Chemical and structural characterization of α-N-acetylgalactosaminidase I and II from starfish, asterina amurensis Deglycosylation and immunostaining of α-GalNAcase I and II. a SDS-PAGE of purified and deglycosylated α-GalNAcase I and II. The proteins were separated on SDS-PAGE and the gel was stained with CBB R-250 solution. S, Molecular weight marker; −, purified enzyme; +, enzyme treated with PNGase F. b Immunostaining between α-GalNAcase and anti-α-GalNAcase antibodies. 1, Antiserum to human α-GalNAcase; 2, antiserum to squid α-GalNAcase I; and 3, antiserum to squid α-GalNAcase II. Lane I, Enzyme α-GalNAcase I; and Lane II, Enzyme α-GalNAcase IIBack to article page