Protein | Km (μM) | kcat (s−1) | kcat/Km (μM−1 s−1) |
---|---|---|---|
Enzymes used in the study | |||
  BfPNK/CPDase (500 ng) | 440 ± 50 | 510 ± 20 | 1.15 |
  ScTrl1 PNK/CPDase (500 ng) | 420 ± 35 | 317 ± 15 | 0.75 |
  ScTrl1 CPDase (500 ng) | 237 ± 27 | 275 ± 18 | 1.16 |
Homologous enzymes | |||
  MmCNPase - full length (500 ng) [30] | 193 ± 26 | 270 ± 10 | 1.40 |
  MmCNPase - catalytic domain (500 ng) [30] | 445 ± 41 | 570 ± 19 | 1.28 |
 Rat CNPase - full length [37] | 263 ± 12 | 836 ± 11 | 3.2 |
 Rat CNPase - catalytic fragment [37] | 295 ± 22 | 1690 ± 39 | 5.7 |