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Table 2 Kinetic parameters for the CPDase activity of different constructs on 2′,3′-cNADP+

From: Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase

Protein

Km (μM)

kcat (s−1)

kcat/Km (μM−1 s−1)

Enzymes used in the study

  BfPNK/CPDase (500 ng)

440 ± 50

510 ± 20

1.15

  ScTrl1 PNK/CPDase (500 ng)

420 ± 35

317 ± 15

0.75

  ScTrl1 CPDase (500 ng)

237 ± 27

275 ± 18

1.16

Homologous enzymes

  MmCNPase - full length (500 ng) [30]

193 ± 26

270 ± 10

1.40

  MmCNPase - catalytic domain (500 ng) [30]

445 ± 41

570 ± 19

1.28

 Rat CNPase - full length [37]

263 ± 12

836 ± 11

3.2

 Rat CNPase - catalytic fragment [37]

295 ± 22

1690 ± 39

5.7