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Fig. 7 | BMC Biochemistry

Fig. 7

From: Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase

Fig. 7

Polynucleotide kinase activity assay. PNK reaction mixtures of a ScTrl1 PNK/CPDase, b BfPNK/CPDase, and c ScTrl1 CPDase. The reactions were carried out in the presence of Mg2+, with ATP as phosphate donor and with either A20, dA20, or both as acceptors. Both ScPNK/CPDase and BfPNK/CPDase display PNK activity with a clear substrate preference. The ScCPDase domain alone is expectedly inactive. The arrow represents the direction of electrophoresis

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BMC Biochemistry

ISSN: 1471-2091

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