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Fig. 6 | BMC Biochemistry

Fig. 6

From: Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase

Fig. 6

Thermal stability assay. a A selection of thermal shift assay melting curves for ScTrl1 PNK/CPDase. Colour code: Violet - 50 mM citric acid, pH 5.5, 150 mM NaCl; Dark red - 50 mM MOPS, pH 6.5, 500 mM NaCl; Orange - 50 mM imidazole, pH 8.0, 150 mM NaCl; Light blue - 50 mM HEPES, pH 7.5, 150 mM NaCl; Green - 50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM 2′,3′-cNADP+. b A selection of thermal shift assay melting curves for Bf PNK/CPDase. Colour code: Dark red - 50 mM MOPS, pH 6.5, 500 mM NaCl; Blue - 50 mM MES, pH 7.0, 150 mM NaCl; Light blue - 50 mM HEPES, pH 7.5150 mM NaCl; Green - 50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM 2′,3′-cNADP+. A clear increase in the melting temperature can be observed, induced by buffer conditions with pH 7.5, low salt concentration, and 2′,3′-cNADP+

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