Fig. 2

Cusabio and Elabscience SIRPA ELISA kits failed to recognize recombinant human SIRPA protein. a Cusabio SIRPA ELISA kit (CSB-EL021334HU) recognized its own calibrator diluted in buffer from Cusabio ELISA kit generating a linear curve (diamonds) but did not recognize recombinant human SIRPA protein (squares); b Elabscience SIRPA ELISA kit (E-EL-H1573) recognized its own calibrator diluted in buffer from Elabscience ELISA kit generating a linear curve (diamonds) but did not recognize recombinant human SIRPA protein (squares). X- axis represents known standard concentration (ng/ml) and Y-axis represents optical density (OD) measured at 450 nm. c Western blot of rhSIRPA using SIRPA antibody (Biolegend, catalog # 323805) detected a ~55 kda band in rhSIRPA but not in calibrators from Cusabio and Elabscience SIRPA ELISA kits