Fig. 2
From: Auto-thiophosphorylation activity of Src tyrosine kinase
Auto-thiophosphorylation activity of other tyrosine kinases. Conditions for the continuous spectrophotometric assay were similar to Fig.Ā 1. The reactions contained enzyme alone (circles), enzyme with 2Ā mM ATPĪ³S and 10Ā mM MgCl2 (squares) or enzyme with 2Ā mM ATPĪ³S and 10Ā mM NiCl2 (triangles). a Hck kinase (0.6Ā Ī¼M). b Ack1 (kinase-SH3 construct), 1.0Ā Ī¼M. c IGF1R kinase domain, 1.4Ā Ī¼M