Enzyme | Vmax (IU/mg) | Km (mM) | Kcat (s−1) | Kcat/Km s−1mM−1 | Kcat/Km (%) |
---|---|---|---|---|---|
Wild-type LacA | 2.61 ± 0.12 | 9.28 ± 0.72 | 21.74 ± 0.99 | 2.35 ± 0.08 | 100 |
LacA-301 V | 0.76 ± 0.02 | 5.57 ± 0.56 | 3.72 ± 0.09 | 0.67 ± 0.08 | 28.65 |
LacA-361Y | 1.03 ± 0.03 | 8.02 ± 0.27 | 8.56 ± 0.24 | 1.07 ± 0.01 | 45.50 |