Fig. 6
Effect of metal ions on nuclease activity of hPLSCR1. a Gel assay: hPLSCR1 was incubated with human genomic DNA in the presence of 10 mM of CaCl2, MgCl2 and ZnCl2 at 37 °C for 60 min and visualized on a 1 % agarose gel. b Metal ions effect on nuclease activity (Kunitz assay): Nuclease activity was calculated from Kunitz assay done for 10 mM of CaCl2, MgCl2 and ZnCl2 or in the absence of metal ions. c Dose dependence of MgCl2 and ZnCl2 on nuclease activity of hPLSCR1. Kunitz assays were performed with 5 mM, 10 mM, 15 mM and 20 mM of MgCl2 and ZnCl2. * denotes statistical significance at p < 0.05, ** denotes statistical significance at p < 0.005; *** denotes statistical significance at p < 0.0005; ns – not significant. Experiments were performed in triplicates and error bars denote standard deviation