Fig. 5

Effect of temperature and pH on nuclease activity of hPLSCR1. a Temperature effect on nuclease activity (gel assay): hPLSCR1(20 pmol) was incubated with human genomic DNA (200 ng) for 60 min at different temperatures such as 25, 30, 37, 40, 45, 50 °C along with a no-enzyme control ‘Control’ and were visualized on a 1 % agarose gel. b Temperature effect on nuclease activity (Kunitz assay): Kunitz assay was performed as described in ‘Methods’ section. The calculated specific activity values from Kunitz assay at various temperatures (25, 30, 37, 40, 45 °C) were plotted. c pH effect on nuclease activity (gel assay): hPLSCR1 (20 pmol) was incubated with human genomic DNA (200 ng) for 60 min at different pH such as 4.5, 5.5, 6.5, 7.5, 8.5, 9.0, 9.5 along with no-enzyme control named ‘Control’ and visualized on a 1 % agarose gel. d pH effect on nuclease activity (Kunitz assay): Kunitz assay was performed as described under ‘Methods’ section. The calculated specific activity values from Kunitz assay at various pH (6.5, 7.5 8.0, 8.5, 9.0, 9.5) were plotted. Experiments were performed at-least three independent times and error bars denote standard deviation