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Fig. 4 | BMC Biochemistry

Fig. 4

From: Identification and characterization of the novel nuclease activity of human phospholipid scramblase 1

Fig. 4

Nuclease activity is protein mediated. a, b Nuclease assay upon protein inactivation: gel assay (a) and Kunitz assay (b) were performed with heat-inactivated and trypsin digested hPLSCR1 (20 pmol) along with native hPLSCR1 (untreated) and a no enzyme control (control). c Effect of hPLSCR1 concentration on nuclease activity (gel assay): 200 ng of human genomic DNA was incubated with various concentrations of hPLSCR1 (2 pmol, 5 pmol, 10 pmol, 15 pmol and 20 pmol) at 37 °C for 60 min and visualized on a 1 % agarose gel stained with 0.5 μg/ml ethidium bromide. ‘Control’ denotes negative control. d Kunitz assay was performed for varying concentrations of hPLSCR1 as described above. * denotes statistical significance at p < 0.05, ** denotes statistical significance at p < 0.005; ns – not significant. Experiments were performed in triplicates and error bars denote standard deviation

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