Fig. 4
From: Molecular characterization of protein kinase C delta (PKCδ)-Smac interactions
Smac can interact with several PKC-isoforms. a, b, c COS-7 cells were transfected with vectors encoding full-length Smac-FLAG and EGFP-fusions of full-length PKC isoforms (a), C1/C2 domains from different isoforms (b) or mutated full-length PKCδ variants carrying a tyrosine-to-aspartate mutation on Tyr-64 (Y64D), Tyr-155 (Y155D) or Tyr-313 (Y313D) (c). After transfection, indicated cells were treated with 16 nM TPA. Lysates were then used for GFP-immunoprecipitation. Figures are representatives from three experiments. Asterisk indicates non-specific band