Fig. 3
From: Molecular characterization of protein kinase C delta (PKCδ)-Smac interactions
Smac binds to the regulatory domain of PKCδ. a Schematic of PKCδ structure. b, c COS-7 cells were transfected with vectors encoding full-length Smac-FLAG together with vectors encoding EGFP-fusions of full-length PKCδ, the regulatory domain or the catalytical domain (b) or different combinations of regulatory domain subregions (c). Lysates were then used for GFP-immunoprecipitation. FL = Full-length, RD = Regulatory domain, CD = Catalytical domain, PS = Pseudosubstrate domain. Figures are representatives from three experiments. Asterisk indicates non-specific band