Linalool isomerase, a membrane-anchored enzyme in the anaerobic monoterpene degradation in Thauera linaloolentis 47Lol

Table 4 Enrichment of Lis activity from spheroplasts of T. linaloolentis 47Lol by sucrose gradient centrifugation

Sample	Protein [mg]	Activity [pkat]	Specific activity [pkat mg⁻¹]	Relative specific activity	Protein yield [%]
F1	40.8	1408	35
F2	111.7	3130	28	1	100.0
F4	94.7	13572	143	5	84.8
F6	58.8	3440	59	2	52.6
SP 4/5	3.7	745	200	7	3.3
Sucrose supernatant	-	-	-	-	-
Sucrose pellet	1.9	323	171	6	1.7

(F1) spheroplasts, (F2) lysed spheroplasts, (F4) crude extract, (F6) cytoplasmatic, soluble protein fraction, (SP 4/5) fractions 4 and 5 from sucrose gradient, (Sucrose supernatant) supernatant after second ultracentrifugation, (Sucrose pellet) protein pellet after second ultracentrifugation

ISSN: 1471-2091