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Table 1 Enrichment of Lis activity from spheroplasts of T. linaloolentis 47Lol

From: Linalool isomerase, a membrane-anchored enzyme in the anaerobic monoterpene degradation in Thauera linaloolentis 47Lol

Sample

Protein [mg]

Activity [pkat]

Specific activity [pkat mg−1]

Relative specific activity

Protein yield [%]*

F1

71.9

7295

101

  

F2

79.0

7800

99

1.0

100.0

F3

67.6

3060

45

0.5

 

F4

51.8

24984

482

4.9

65.6

F5

29.1

513

18

0.2

36.8

F6

38.0

3283

86

0.9

73.3

F7

21.4

15848

741

7.5

41.3

  1. (F1) spheroplasts, (F2) lysed spheroplasts, (F3) outer membrane and periplasmatic proteins, (F4) crude extract from spheroplasts, (F5) unbroken spheroplast and cell debris, (F6) cytoplasmatic, soluble proteins, (F7) inner membrane fraction. *F4 and F5 are derived from F2. F6 and F7 are derived from F4