Purification step | Total Cyt c (mg) | Purity (OD410/280) | Recovery (%) | Fold purification |
---|---|---|---|---|
Lysate | 43.9 ± 1.2 | 0.223 ± 0.005 | 100 | 1.00 |
Ammonium Sulphate (Precipitation) | 30.8 ± 0.7 | 0.306 ± 0.001 | 70.2 | 1.37 |
Dialysis | 20.7 ± 0.2 | 0.440 ± 0.001 | 47.2 | 1.97 |
Cation exchange | 12.4 ± 0.3 | 3.91 ± 0.10 | 28.2 | 17.50 |
Gel filtration | 9.12 ± 0.13 | 4.26 ± 0.03 | 19.7 | 19.10 |