Identification of the minimum fragment from polyhedrin that retains the self-aggregation property.
A, confocal microscopy studies using the fragments PH(1–58)EGFP, PH(58–110)EGFP and PH(1–110)EGFP alone, or in co-expression with WT polyhedrin (B). Boxes below each panel indicate cellular localization based on the degree of co-localization with the nuclear marker DAPI. Notice that fragment PH(1–58)EGFP is soluble and found in both the nucleus and the cytosol. Fragments PH(58–110)EGFP and PH(1–110)EGFP can form self-aggregates when expressed alone or be incorporated into polyhedra, when co-expressed with WT polyhedrin. Thus the minimum self-aggregating fragment identified in this study was PH(58–110)EGFP.