Titration of the amount of WT polyhedrin required to integrate
into the polyhedra crystal.
A, upper panels show the fluorescence of PH(1–245)-EGFP and DAPI, while lower panels show differential interference contrast (DIC) in cells co-infected with baculoviruses carrying PH(1–245)-EGFP (multiplicity of infection , MOI =1) and a second baculovirus carrying a wild type copy of polyhedrin. The second baculovirus was utilized at increasing MOIs of 0.5, 1, 2, 3, 4 and 5 (only 4 MOIs shown for illustration purposes). Notice that at a MOI of 3 (and above), all PH(1–245)-EGFP was contained inside the polyhedra crystals. B, percentage of pixel co-localization between EGFP and DAPI, to determine the amount of PH(1–245)-EGFP present inside the nucleus. Notice that with a MOI of 3, all PH(1–245)-EGFP localizes in the nucleus (no differences were observed between MOIs of 3 and 5). C, polyhedra containing PH(1–245)-EGFP were purified by centrifugation from Sf9 insect cells subjected to sonication. Notice that all polyhedra were fluorescent, indicating that PH(1–245)-EGFP was present.