Figure 1

Identification of different motifs for nuclear localization, self-aggregation and incorporation into polyhedra in polyhedrin. A, diagram illustrating the different fragments from the polyhedrin protein tested in this study. In red as depicted the fragments that are not incorporated into the polyhedra crystal (when co-infected with a virus carrying a copy of WT polyhedrin). Inside the rectangles are indicated the amino acid numbers for the different fragments. B, analysis of the secondary structure of wild type polyhedrin, obtained from the crystallographic structure (2WUY.pdb, http://www.rcsb.org/pdb/explore.do?structureId=2wuy)). Red barrel depict α helices and green cubes β sheets, while coils are depicted as straight lines. C, identification of the self-aggregating fragments from polyhedrin and its cellular localization. Red indicates only nuclear and yellow nuclear and cytosolic. D, diagram indicating the self-aggregating domain and nuclear localization domain in the N-terminal region from polyhedrin. Domains are shaded in color for easier identification.