From: Protein expression, characterization and activity comparisons of wild type and mutant DUSP5 proteins
M-M Kinetics | GST-DUSP5 | GST-S147P | GST | DUSP5-PD |
---|---|---|---|---|
Vmax (nmol/min) | 0.63 ± 0.03 | 0.27 ± 0.01 | NA | 0.06 ± 0.01 |
Km (mM) | 5.24 ± 1.0 | 2.33 ± 0.56 | NA | 1.7 ± 0.45 |
M-M Kinetics | DUSP5 (cleaved) | S147P (cleaved) | Â | Â |
Vmax (nmol/min) | 0.36 ± 0.03 | 0.13 ± 0.01 |  |  |
Km (mM) | 3.0 ± 1.0 | 1.3 ± 0.55 |  |  |