Skip to main content

Table 1 Michaelis-Menten parameters (Non-linear least squares regression)

From: Protein expression, characterization and activity comparisons of wild type and mutant DUSP5 proteins

M-M Kinetics

GST-DUSP5

GST-S147P

GST

DUSP5-PD

Vmax (nmol/min)

0.63 ± 0.03

0.27 ± 0.01

NA

0.06 ± 0.01

Km (mM)

5.24 ± 1.0

2.33 ± 0.56

NA

1.7 ± 0.45

M-M Kinetics

DUSP5 (cleaved)

S147P (cleaved)

  

Vmax (nmol/min)

0.36 ± 0.03

0.13 ± 0.01

  

Km (mM)

3.0 ± 1.0

1.3 ± 0.55

 Â