Figure 2
From: Protein expression, characterization and activity comparisons of wild type and mutant DUSP5 proteins
GST-DUSP5 and GST-S147P Proteins Purification. (A) GST-DUSP5 protein purification. Lane 1: MW marker, lane 2: cell lysate, lane 3: GST pull down (GST-DUSP5 was adsorbed on glutathione sepharose 4B and loaded on gel), lanes 4–7 purified GST-DUSP5 protein fractions. (B) GST-DUSP5 PreScission protease cleavage. Lane 1: MW marker, lane 2: Purified GST-DUSP5, lanes 3–6: GST-DUSP5 protein eluted following PreScission protease digestion. (C) GST-S147P protein purification. Lane 1: MW marker, lane 2: cell lysate, lane 3: GST pull down (GST-S147P was adsorbed on glutathione sepharose 4B and loaded on gel), lanes 4–7 purified GST-S147P protein fractions. (D) GST-S147P PreScission protease cleavage. Lane 1: MW marker, lane 2: purified GST-S147P, lane 3–6: protein eluted following PreScission protease digestion.