Figure 4From: Integrated allosteric regulation in the S. cerevisiae carbamylphosphate synthetase – aspartate transcarbamylase multifunctional proteinSubstrate saturation curves of the CPSase from the mutant Glu 2182→Lys in presence or absence of UTP. The CPSase activity was measured as indicated in the Methods. On the left are represented the double reciprocal plots in the presence of (●) 0 mM, (◆) 3 mM, and (■) 6 mM UTP. On the right are presented the corresponding Dixon plots. The concentrations used were (●) 6.7 mM, (◆) 9.3 mM, (■) 10.7 mM, and (○) 33 mM in the case of ATP; (●) 2.1 mM, (◆) 5.3 mM, (■) 7.5 mM, and (○) 20 mM in the case of bicarbonate; (●) 0.03 mM, (◆) 0.07 mM, (■) 0.3 mM, and (○) 1 mM in the case of glutamine.Back to article page