Figure 10

Probable interface between the CPSase and ATCase domains of the S. cerevisiae bifunctional complex. In green: the yeast CPSase domain. In red: the yeast ATCase domain. In yellow: point mutations which specifically abolish the sensitivity of the ATCase activity to UTP (see Table 1). From top to bottom: Asp1220, Trp1112, Tyr1096, Asn1094, Arg1076 in the CPSase domain; Glu1933, Glu2052, Phe1924, Asn2058, Asp2186, Glu2182, Asn2157 in the ATCase domain. The CPSase catalytic subsites are indicated by two molecules of ADP.