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Figure 8 | BMC Biochemistry

Figure 8

From: Importance of extended protease substrate recognition motifs in steering BNIP-2 cleavage by human and mouse granzymes B

Figure 8

GrB induced BNIP-2 cleavage in cellulo . Human and mouse GrB delivered by means of SLO to human HeLa and murine NIH/3T3 cells transfected with hBNIP-2 and mBNIP-2 respectively were examined for in cellulo BNIP-2 cleavage. The in vitro (IV) hGrB induced cleavage pattern of hBNIP-2 in transfected HeLa cell lysates as well as HeLa and NIH/3T3 cells transfected with the truncated hBNIP-2 and mBNIP-2 variants respectively are included for comparison. Black arrows indicate BNIP-2 precursor patterns whereas dashed and grey arrows are indicative for BNIP-2 cleavage fragments.

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BMC Biochemistry

ISSN: 1471-2091

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