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Figure 4 | BMC Biochemistry

Figure 4

From: Importance of extended protease substrate recognition motifs in steering BNIP-2 cleavage by human and mouse granzymes B

Figure 4

Granzyme B mediated cleavage of a peptide substrate holding the hBNIP-2 cleavage motif. Cleavage progression of the hBNIP-2 peptide substrate NH2.LPEDDSIEADILAITGY(NO2)R.OH was assayed for 2 h at 37°C with various concentrations of human and mouse GrB (50, 200 and 500 nM) at a substrate concentration of 100 μM, and its precursor and fragment peptides separated by RP-HPLC (chromatograms with absorbance at 214 nm are shown). The grey arrow indicates (residual) precursor peptide, whereas black and dashed arrows indicate peptide fragments generated upon human (upper panel) and mouse (lower panel) GrB mediated cleavage at IEAD (the dashed arrow corresponds to the N-terminal fragment NH2.LPEDDSIEAD.OH, whereas the black arrow corresponds to the C-terminal fragment NH2.ILAITGY(NO2)R.OH).

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