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Figure 3 | BMC Biochemistry

Figure 3

From: Importance of extended protease substrate recognition motifs in steering BNIP-2 cleavage by human and mouse granzymes B

Figure 3

Probing granzyme B cleavage efficiencies of Bid and BNIP-2 in cell-free lysates. Alkylated freeze-thaw lysates of human Jurkat and mouse YAC-1 cells were incubated with serial dilutions (ranging from 1 μM to 0.97 nM (from right to left)) of human and mouse granzyme B to assess BNIP-2 (A: Jurkat) and Bid (B: Jurkat and C: YAC-1) cleavage. Precursors are indicated by black arrows, whereas dashed and grey arrows represent GrB generated BNIP-2 cleavage fragments. BNIP-2 results in the YAC-1 background are lacking since no endogenous BNIP-2 precursors could be detected by means of immunoblotting (data not shown).

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BMC Biochemistry

ISSN: 1471-2091

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