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Figure 5 | BMC Biochemistry

Figure 5

From: Expression and characterization of a β-fructofuranosidase from the parasitic protist Trichomonas vaginalis

Figure 5

Hydrolysis of sucrose by T. vaginalis β-fructofuranosidase. Purified β-fructofuranosidase (~27 nM, final concentration) was incubated under standard assay conditions with the indicated concentrations of sucrose and the initial rate of sucrose hydrolysis was measured. Data were fitted to the Michaelis-Menten equation in order to determine kinetic constants. The results shown are the mean ± standard error for three independent experiments. Km = 20.6 ± 2.2 mM; Vmax = 1576 ± 48 μmol/min/mg.

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