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Table 1 Fractions of F 2 FlAsH and its complexes’ fluorescence with a lifetime of 1 ns

From: Biarsenical ligands bind to endogenous G-protein α-subunits and enable allosteric sensing of nucleotide binding

 

Fraction τ(1.0 ns)a%

χ2

F2FlAsH

34 ± 3

1.9

F2FlAsH + GTPγS

29 ± 1

3.7

CCPGCC-peptide

11 ± 2

3.8

βγM

25 ± 2

4.6

αolf

12 ± 5

1.67

αolf + GTPγS

56 ± 4b

12b

α13

37 ± 4

6.7

α13 + GTPγS

38 ± 3

2.5

  1. aFluorescence lifetimes were fitted to equations (1–3) with two components (1.0 ± 0.2 ns and 4.3 ± 0.1 ns) and fractions corresponding to 1.0 ns are reported as mean ± SD.
  2. bA better fit was obtained by including a third very fast component corresponding to 17 % of fluorescence, but with a lifetime that was below the measurement range of the instrument (τ < 0.1 ns).
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BMC Biochemistry

ISSN: 1471-2091

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