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Table 1 Differentially expressed proteins in Vitamin C treated AGS cells identified by MALDI-TOF/MS

From: Proteomic analysis of differentially expressed proteins in vitamin C-treated AGS cells

Spot no.

Swissprot entry name1

Protein name1

Accession number1

Theoretical/ experimental Mr (kDa)2

Theoretical/ experimental pI value2

Protein score3

Sequence coverage (%)/peptides matched

Fold change

Up - regulated proteins

2

TNIP2_HUMAN

TNFAIP3-interacting protein 2

Q8NFZ5

49.24/91

6.03/5.83

56

15/7

2.3

3

XIRP1_HUMAN

Xin actin-binding repeat-containing protein 1

Q702N8

199.64/96

5.78/6.48

59

11/13

2.2

6

TXND5_HUMAN

Thioredoxin domain-containing protein 5

Q8NBS9

48.28/49

5.63/5.38

81

22/10

2.9

7

CE290_HUMAN

Centrosomal protein of 290 kDa

O15078

290.89/28

5.75/6.04

69

9/16

8.5

8

PRDX4_HUMAN

Peroxiredoxin-4

Q13162

30.75/24

5.86/5.43

68

38/6

3.0

9

CE290_HUMAN

Centrosomal protein of 290 kDa

O15078

290.89/23

5.75/5.21

60

8/15

6.4

Down - regulated proteins

1

DPP3_HUMAN

Dipeptidyl peptidase 3

Q9NY33

82.88/100

5.02/4.86

110

26/12

4.0

4

DCTN2_HUMAN

Dynactin subunit 2

Q13561

44.32/51

5.10/4.94

122

39/13

2.3

5

IF4A1_HUMAN

Eukaryotic initiation factor 4A-I

P60842

46.35/48

5.32/5.31

175

47/17

2.1

10

PSB6_HUMAN

Proteasome subunit beta type-6

PSMB6

25.57/21

4.80/4.64

104

39/10

4.4

11

EIF3K_HUMAN

Eukaryotic translation initiation factor 3 subunit K

Q9UBQ5

25.33/21

4.81/4.46

101

37/8

3.6

12

PSA5_HUMAN

Proteasome subunit alpha type-5

P28066

26.57/24

4.74/4.31

91

41/8

2.0

13

IF6_HUMAN

Eukaryotic translation initiation factor 6

P56537

27.10/23

4.56/4.13

86

50/7

3.8

14

1433Z_HUMAN

14-3-3 protein zeta/delta

P63104

27.9/25

4.73/4.36

93

40/10

2.6

15

1433S_HUMAN

14-3-3 protein sigma

P31947

27.87/25

4.68/4.26

150

58/12

2.2

16

1433E_HUMAN

14-3-3 protein epsilon

P62258

29.33/26

4.63/4.27

131

93/12

2.3

17

EF1B_HUMAN

Elongation factor 1-beta

P24534

24.92/26

4.50/4.12

130

50/11

3.3

18

TPM4_HUMAN

Tropomyosin alpha-4 chain

P67936

28.62/27

4.67/4.28

94

25/10

3.1

19

TPM3_HUMAN

Tropomyosin alpha-3 chain

P06753

32.86/27

4.68/4.37

95

20/11

2.7

20

PCNA_HUMAN

Proliferating cell nuclear antigen

P12004

29.09/29

4.57/4.28

127

40/10

2.5

  1. 1Entry name, protein name and accession number from SWISS-PROT database identified by MALDI-TOF/MS.
  2. 2Theoretical molecular weight (kDa) and pI from SWISS-PROT database; the experimental values of pI and MW for every isoelectric spot were calculated using Progenesis samespots software (Nonlinear Dynamics Ltd., Newcastle, UK).
  3. 3Score is −10*log (p), where p is the probability that the observed match is a random event, Protein scores greater than 56 are significant (p<0.05).
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BMC Biochemistry

ISSN: 1471-2091

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