From: Phosphatidylcholine formation by LPCAT1 is regulated by Ca2+ and the redox status of the cell
Purpose | Primer sequence (5′ to 3′) |
---|---|
saturated mutagenesis of | GCCTCAGATAATGATTTTTCCAGAAGGAACTNNKACAAA- |
Cys-211 codon | TAGGACCTGCCTCA |
Cys-211 to Ser | GATTTTTCCAGAAGGAACTAGTACAAATAGGACCTGCCT |
Glu-403 to Ala | TGAGATTGACCTTCGTGCATATGTGGTCGCCTTGT |
Cys-216 to Ala | GAACTGCTACAAATAGGACCGCCCTCATTACCTTCAAACCTG |
Cys-263 to Ala | AATCCTGTGGCTCACTCTGGCCCAGTTTCAAAACCAAGTG |
Cys-278 to Ala | GTGGAAATTGAATTTCTGCCTGTGTATGCCCCTTCTGAAGAGG |
Cys-314 to Ala | ACTATACATTTGAGGACGCCCAGCTGGCTCTGGCAG |
Cys-330 to Ala | CTTGCCTGCTGACACCGCCCTGCTAGAGTTTGCC |
Cys-401 to Ala | GCCTTGTCTGTGGTGGCCAGGCCATCCCAGAC |
Cys-443 to Ala | GAGGCCAACCTGTCCGCCATCCTCAAGACTGC |
Cys-477 to Ala | AATCACCTTTGATGACTTCGCCGGGTTTGCGGAAATGTAC |
Cys-501 to Ala | GACACATTTCGACAGCGCTGCACAGACACCCCCA |
Cys-514 to Ala | CAACTCCCAATGGCTTCGCCATTGACTTCAGCCCTG |
Cys-530 to Ala | ACTTTGGGAGAAAGAATTCTGCTAAGAAAGCGGACTAGCCTC |