Table 1 Kinetic analysis of in vitro lipid kinase activity of p110 αEE^WT/p85 α, p110 αEE^E545K/p85 α and p110 αEE^H1047R/p85 α

PI kinase activity ^a

V _max ^b

35 ± 13^c

118 ± 12

150 ± 13

K _m (μM)

23 ± 3

22 ± 6

14 ± 7

PI-(4,5)-P ₂ kinase activity

V _max

35 ± 8

117 ± 13

146 ± 15

K _m (μM)

22 ± 5

19 ± 6

9 ± 3