Figure 8
From: Presenilin-1 regulates the constitutive turnover of the fibronectin matrix in endothelial cells
The fibrillar state of fibronectin is increased in PS1−/− endothelial cells. Passage 3 wild type and PS1−/− endothelial cells were grown to confluency in fibronectin-depleted medium. The endothelial cells were lysed with deoxycholate (DOC) lysis buffer and DOC-insoluble material was isolated by centrifugation and analyzed by Western blot. In panel A, blots of DOC-insoluble and soluble material were sequentially probed for fibronectin (FN) and vimentin (vim) or fibronectin and β-tubulin (β-tub). Panel B shows quantitation of the levels of DOC insoluble fibronectin in the experiment shown in panel A. In panel C, the ratio of DOC insoluble/DOC soluble fibronectin is shown. Asterisk indicates p < 0.05 vs. wild type (unpaired t-test). 1 μg of protein was loaded per lane. A representative blot is shown from experiments that were performed twice.