Figure 5
From: The role of the C8 proton of ATP in the catalysis of shikimate kinase and adenylate kinase
Adenylate kinase specific activity of WT, T23V and R128A mutant enzymes. The effect of the concentration of ATP and C8D-ATP on the specific activity of human adenylate kinase. ○ = WT AK1 using ATP, □ = WT AK1 using C8D-ATP, ● = T23V mutant using ATP, ♦ = T23V mutant using C8D-ATP, ▲ = R128A mutant using ATP, Δ = R128A mutant using C8D-ATP. R128A data on right axis. Final enzyme concentrations were: WT AK and T23V: 0.33 mM and R128A: 650 nM. The assays were run for 95 min (WT AK and T23V) or 80 min (R128A).