Figure 8
From: Roles of quaternary structure and cysteine residues in the activity of human serine racemase
Effect of chemical reduction on quaternary structure of recombinant SR: denaturing conditions. A. Recombinant SR was dialyzed against phosphate buffers with or without 0.2 mM DTT after elution from the nickel affinity column. B. SR preparations in panel A were injected onto a Superdex HR200 column and eluted with same buffer as the corresponding dialysis buffer. Recombinant SR oligomeric species of both protein preparations in A and B were subjected to SDS-PAGE in presence or absence of 0.2 mM DTT in the sample loading buffer. The gels were then immunoblotted with anti-SR antibody.