Figure 7
From: Roles of quaternary structure and cysteine residues in the activity of human serine racemase
Effect of chemical reduction on quaternary structure of recombinant SR: native conditions. Recombinant SR purified by affinity chromatography was dialyzed against the phosphate buffer with (dashed line) or without (solid line) 0.2 mM DTT. After concentration with Centricon filters, 50-μl aliquots of ~10 mg/ml of each recombinant SR were injected onto a Superdex 200 column and eluted with the same buffer as the dialysis buffer.