Figure 4From: Roles of quaternary structure and cysteine residues in the activity of human serine racemaseInfluence of buffer components on SR activity. Recombinant SR purified by affinity chromatography was dialyzed against a phosphate-buffered (solid line) or Tris-buffered solution (dashed line). Then, SR activity of each enzyme preparation was monitored as described under "Methods" except for the reaction buffers, which corresponded to each respective dialysis buffer.Back to article page