Figure 2
From: Roles of quaternary structure and cysteine residues in the activity of human serine racemase
Gel-filtration chromatography of recombinant SR. Purified recombinant SR with affinity column was extensively dialyzed against 50 mM phosphate buffer, pH 8.0, 52 mM NaCl. After concentration with Centricon filters, aliquots of 50 μl of ~3 mg/ml of recombinant SR were loaded onto a Superdex HR200 gel-filtration column. Separation was performed at 25°C with flow rate at 0.5 ml/min. The elution buffer was the same as dialysis buffer. Protein detection was performed at 280 nm.