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Table 2 Summary of MALLS and AUC experiments

From: The major leucyl aminopeptidase of Trypanosoma cruzi (LAPTc) assembles into a homohexamer and belongs to the M17 family of metallopeptidases

SEC-MALLS

Calibrated SEC-AUC

N° of subunits

Elution volume (ml)

Recovery mass fraction (%)

Molecular mass (kDa)

Stoke radius (nm)

Sedimentation coefficient (S)

Molecular mass (kDa)

 

8.5

11

1025

ND

19.5

ND

17.5a

9

13

625

6.8

15.3

593

10.1b

10

45

314

5.7

10.2

330

5.6b

11.2

4

176

ND

5.1

ND

3a

  1. a From SEC-MALLS; b From SEC-AUC