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Figure 3 | BMC Biochemistry

Figure 3

From: The major leucyl aminopeptidase of Trypanosoma cruzi (LAPTc) assembles into a homohexamer and belongs to the M17 family of metallopeptidases

Figure 3

LAPTc assembles into a hexamer. (A), SEC-MALLS data obtained with 20 μl rLAPTc at 10 mg/ml (170 μM). Superposition of concentration signal (differential refractive index, continuous line), light scattering signal (dotted line) and molecular mass calculation (discontinuous line). For clarity, the differential refractive index and light scattering signals are normalized. (B), superposition of experimental (dots) and fitted (continuous line) AUC profiles corrected for all systematic noise for LAPTc at 56 μM (upper panel). Superposition of the differences between the experimental and fitted curves (lower panel). The fit was obtained from the c(s) analysis of the program SEDFIT. (C), corresponding c(s) distribution in the range 1-45 S.

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BMC Biochemistry

ISSN: 1471-2091

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